DOG1 Antibody Cocktail / TMEM16A / ANO1 orb385747 from Biorbyt

Description

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%

Product Specs
ItemDOG1 Antibody Cocktail / TMEM16A / ANO1
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb385747
Quantity20 µg, 100 µg
ApplicationsIHC-P
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman
TargetDOG1
IsotypeMouse IgG1, kappa
CloneDG1/447 + DOG-1.1
HostMouse
ImmunogenRecombinant human DOG1 protein (DG1/447) and amino acids 904-986 (DOG-1.1) were used as the immunogens for this DOG-1 antibody cocktail
NCBI Full Gene Nameanoctamin 1
NCBI Gene AliasesDOG1, INDMS, ORAOV2, TAOS2, TMEM16A
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the DOG-1 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The optimal dilution of the DOG-1 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

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